Crystallization of agglutinin from the seeds of Abrus precatorius

Agglutinin protein purified from the seeds of Abrus precatorius has a high antitumour activity and was crystallized at room temperature with polyethyl ene glycol 8000 as the precipitant. The agglutinin crystal diffracted to 3. 45 Angstrom and belongs to one of two possible tetragonal space groups, P4( 1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 141.91, c = 105.63 Angstrom. The asymmetric unit contains a heterotetrameric protein molecule of molecular weight 134 kDa and has a solvent content of approximately 38% .