Although motorways could affect wildlife species, only few studies have bee
n documented on their effects on mortality and isolation. With 2266 road-ki
lled animals representing 97 species, the results of a study on a motorway
section emphasized that traffic considerably affected vertebrate population
s (14.5 animals day(-1) 100 km(-1)). Road-killed animals were mainly mammal
s (43.2%), with predators also suffering critical impacts (21.7% vertebrate
s). Rare or endangered species such as the Midwife toad, the Blue throat, t
he little Horseshoe bat, or the European otter were among the victims. Anim
al mortality exponentially increased with traffic volume. Mortality reached
almost 100% of migrants when no passage existed, and this barrier effect w
as only reduced when underground passages crossed the road restraining the
mortality to 31% of migrants in Field mice and 23% in Common toads, while m
ortality always exceeded 74% in a road section with fauna ducts. It is reas
onable to conclude that traffic severely influenced both wildlife species d
emography and population exchanges resulting in effective population isolat
ion.