The effects of thiopentone on free intracellular amino acids in polymorphonuclear leucocytes

Previous studies have shown the inhibitory effects of thiopentone on polymo rphonuclear leucocyte (PML) function. However, major biochemical mechanisms which have been involved are still unknown. The aim of this study was ther efore to investigate thiopentone's effects on intracellular amino acid meta bolism in PML using both advanced PML separation - and HPLC techniques, esp ecially developed for this purpose and precisely validated in our institute . Overall, our study indicates important dose-dependent alterations of free intracellular amino acid metabolism following thiopentone treatment and dr aw attention to the biochemical mechanisms which may be involved in both th iopentone-induced modulation in PML function and cellular immunocompetence.