Threshold and innervation density tests are common clinical tools used in t
he evaluation of peripheral nerve injuries and compression syndromes. The p
urpose of this study is to determine the inter- and intraobserver reliabili
ty of Semmes-Weinstein monofilaments and static and moving two-point discri
mination in 48 volunteers. Kappa coefficients of inter- and intraexaminer a
greement were generated for each test and investigator. The interexaminer r
eliability for the Semmes-Weinstein monofilaments ranged from fair to moder
ate in the ulnar and median nerve distributions, and slight to moderate in
static and moving two-point discrimination testing. Intraobserver reliabili
ty for Semmes-Weinstein monofilaments and static and moving two-point discr
imination was slight to fair for both examiners. Our data indicate that Sem
mes-Weinstein monofilaments and two-point discrimination tests yield unreli
able measurements in asymptomatic individuals. Although useful in monitorin
g neurological function in pathological states, threshold and innervation d
ensity measurements from an unaffected digit or extremity may not represent
a reliable standard for comparison of abnormal values.