The water masers in NGC 4258 delineate the structure and dynamics of a subp
arsec-diameter accretion disk around a supermassive black hole. Very Long B
aseline Array (VLBA) observations provide precise information about the pos
itions in the plane of the sky and the three-dimensional velocity vectors f
or the maser emission, but the positions along the line of sight must be in
ferred from models. Previous measurements placed an upper limit on the acce
lerations of the high-velocity spectral features of 1 km s(-1) yr(-1), sugg
esting that they are located near the midline (the diameter perpendicular t
o the line of sight), where they would have exactly zero acceleration. From
similar measurements, the accelerations of the systemic velocity spectral
features have been estimated to be about 9 km s(-1) yr(-1), indicating that
they lie toward the front of the disk where the acceleration vector points
directly away from the line of sight. We report acceleration measurements
for 12 systemic velocity spectral features and 19 high-velocity spectral fe
atures using a total of 25 epochs of observations from Effelsberg (five epo
chs), the Very Large Array (15 epochs), and the VLBA (five epochs) spanning
the years 1994-1997. The measured accelerations of the systemic velocity f
eatures are between 7.5 and 10.4 km s(-1) yr(-1), and there is no evidence
for a dip in the spectrum at the systemic velocity. Such a dip has been att
ributed in the past to an absorbing layer of noninverted H2O. The accelerat
ions of the high-velocity features, measured here for the first time, range
from -0.77 to 0.38 km s(-1) yr-'. From the line-of-sight accelerations and
velocities, we infer the positions of these high-velocity masers with a si
mple edge-on disk model The resulting positions fall. between -13 degrees 6
and 9 degrees 3 in azimuth (measured from the midline). A model that sugge
sts a spiral shock origin of the masers, in which changes in maser velocity
are due to the outward motion of the shock wave, predicts apparent acceler
ations of -0.05(theta(p)/2 degrees 5) km s(-1) yr(-1), where theta(p) is th
e pitch angle of the spiral arms. Our data are not consistent with these pr
edictions. We also discuss the physical properties of the high-velocity mas
ers. Most notably, the strongest high-velocity masers lie near the midline,
where the velocity gradient is smallest, thereby providing the longest amp
lification path lengths.