Determination of Ibuprofen in human plasma by high-performance liquid chromatography: validation and application in pharmacokinetic study

A specific method for the simultaneous determination of S-(+)Ibuprofen and R-(-)Ibuprofen enantiomers in human plasma is described. Adopting a high-pe rformance liquid chromatographic (HPLC) system with spectrofluorometer dete ctor, the compounds were extracted from plasma in alcohol medium and were s eparated on C18 column, using a solution of acetonitrile-water-acetic acid- triethylamine as mobile phase. The limit of quantitation was 0.1 mu g/mL fo r both compounds. The method was validated by intra-day assays at three con centration levels and was used in a kinetic study in healthy volunteers. Du ring the study we carried out inter-day assays to confirm the feasibility o f the method. Copyright (C) 2000 John Wiley & Sons, Ltd.