Enantioselective HPLC analysis of propafenone and of its main metabolites using polysaccharide and protein-based chiral stationary phases

HPLC on chiral stationary phases has been used for the enantioselective ass ay of propafenone (PPF), 5-hydroxypropafenone (PPF-50H) and N-despropylprop afenone (PPF-NOR) enantiomers. The results obtained on Chiralpak AD column showed that it is useful for the resolution of PPF and of its main metaboli tes, although the peaks obtained for PPF-NOR were not symmetrical under the conditions investigated. This column and circular dichroism-based detectio n system were used to determine the absolute configuration of the eluates. Furthermore, the influence of the mobile phase composition on the resolutio n of PPF and of its main metabolites was investigated on cellulose derivati ves (Chiralcel OD-H and Chiralcel OD-R) and protein (Chiral AGP and Ultron ES-OVM)-based chiral stationary phases. The enantiomers of PPF were resolve d on all the columns, except for the Ultron ES-OVM. This column, the Chiral pak AD and the Chiralcel OD-H columns were suitable for the resolution of t he PPF-50H enantiomers. The PPF-NOR enantiomers were resolved on the Chiral pak AD, Chiral AGP and Chiralcel OD-R columns. Copyright (C) 2000 John Wile y & Sons, Ltd.