Enantioselective determination of bromoisovalerylurea by liquid chromatography on chiral stationary phase in reversed- or normal-phase partition mode

Citation
T. Nishikawa et al., Enantioselective determination of bromoisovalerylurea by liquid chromatography on chiral stationary phase in reversed- or normal-phase partition mode, BIOMED CHRO, 14(4), 2000, pp. 243-248
Citations number
9
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOMEDICAL CHROMATOGRAPHY
ISSN journal
02693879 → ACNP
Volume
14
Issue
4
Year of publication
2000
Pages
243 - 248
Database
ISI
SICI code
0269-3879(200006)14:4<243:EDOBBL>2.0.ZU;2-6
Abstract
Bromoisovalerylurea (bromvalerylurea) is a sedative-hypnotic given orally a s a racemate. Enantiomers of this drug could be separated by high-performan ce liquid chromatography on the three chiral stationary phases (a vancomyci n-bonded, beta-cyclodextrin derivative-bonded, or urea derivative-bonded ph ase). Biological fluids of human subjects who had ingested toxic or therape utic doses of the racemate were chromatographed after liquid-liquid extract ion. The (+)-enantiomer concentration was almost equal to the (-)-enantiome r concentration in the serum of one overdosed patient. In all the other sub jects, the (+)-enantiomer was less than the (-)-enantiomer in their sera an d saliva. The data suggest that the drug is absorbed non-stereoselectively from the gasrrointestinal trace and eliminated from the blood stereoselecti vely. Copyright (C) 2000 John Wiley Br Sons, Ltd.