Habitat features and ostracod populations were monitored monthly between De
cember 1995 and September 1996 in three contiguous pools of a Mediterranean
helocrene spring. Principal-components analysis (PCA) of the physical and
chemical properties of the water showed that seasonal variations were minor
in the site closest to the source (pool 1). Conversely, the site the most
distant from the source (pool 3) had the greatest variation in temperature
and oxygen and calcium contents. Differences in limnological factors, espec
ially the equilibrium system of carbonates, among sites were also clear fro
m the results obtained with PCA. The cosmopolitan ostracod Cypridopsis vidu
a predominated in pool 1, where variations in oxygen content and temperatur
e were minimal, and showed a univoltine reproductive pattern. The crenophil
ous species Potamocypris villosa and Herpetocypris intermedia were the most
abundant ostracods at the other two sites, where the development of filame
ntous algae and Characeae produced a high oxygen concentration and an incre
ase in calcium carbonate precipitation. The latter two species both produce
d two generations per year. The small size of springs and their position be
tween interstitial and surface waters make them excellent systems for the s
tudy of ostracod ecology and the influences of environmental factors upon t
heir life-history traits.