HPLC enantioseparation of amino acids and their protected derivatives usedin peptide synthesis with pre-column chiral derivatization

Indirect chiral separation methods based on enantiomeric derivatizations we re developed in order to monitor optical purity of uncoded amino acids and a new series of amino acid derivatives. Marfey's reagent was used for deriv atization of amino groups: whilst boxyl groups were derivatised with (1R, 2 R)- or (1S, 2S)-2-amino-1(4-nitrophenyl)-1,3-propanediol reagents were used , respectively. The separations of diastereomeric derivatives formed via de rivatization were optimized in RP-HPLC and NP-HPLC systems.