Determination of ambroxol in dog plasma by high-performance liquid chromatography and UV detection

A new sensitive HPLC-UV method has been developed and validated for the det ermination of ambroxol in dog plasma enabling the investigation of a newly developed 75 mg ambroxol-containing retard capsule of EGIS Pharmaceuticals Ltd., Budapest, Hungary. A gradient method was used for removing the longer retained plasma components of no interest. The separation was performed on a BDS Hypersil C18 (5 mu m, 250 x 2.1 mm) analytical column, supplied with a 10 mm guard column containing the same packing material. The detection w as performed at 210 nm. The calibration curve was linear in the range 25-20 00 ng . mL(-1). Nerisopam (EGIS-6775) was used as internal standard.