Unravelling genetic data by arrayed primer extension

Authors
Tonisson, N Kurg, A Kaasik, K Lohmussaar, E Metspalu, A
Citation
N. Tonisson et al., Unravelling genetic data by arrayed primer extension, CLIN CH L M, 38(2), 2000, pp. 165-170
Citations number
27
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
ISSN journal
14346621 → ACNP
Volume
38
Issue
2
Year of publication
2000
Pages
165 - 170
Database
ISI
SICI code
1434-6621(200002)38:2<165:UGDBAP>2.0.ZU;2-5
Abstract
We have developed a method for arrayed primer extension (APEX) on an oligon ucleotide microchip together with the 4-color fluoresence imaging equipment and supporting software, that allows analysis of the DNA sequence and chan ges in it. Mutation analysis of BRCA1 gene and single nucleotide polymorphi sm (SNP) chip for genotyping were used as a model system. Chip surface chem istry, template preparation and APEX reaction conditions were optimised and the assay is ready to be implemented in variety of DNA analysis from SNP t esting to DMA resequencing.