Mm. Tedesco et al., Chiroptical rhythmicity, part 3: implications on the activity of the functional cell-surface receptor model, COLL SURF A, 169(1-3), 2000, pp. 5-11
Citations number
25
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
The effect of ligand-induced rhythmical structural changes of the artificia
l receptor 1 on its capacity to mediate ion transport across lipid bilayer
membranes was studied. The changes of the intravesicular pH of egg yolk pho
sphatidylcholine-small unilamellar vesicles (EYPC-SUVs) after application o
f a transmembrane proton gradient were measured by double-channel fluoresce
nce kinetics in the presence of 1 at various concentrations of the extraves
icular ligand L-His. Comparison with negative control experiments using D-H
is indicated that increasing concentrations of L-His affect the activity of
1 in a presumably rhythmical manner. Compared to the extent of structural
rhythmicity, the functional rhythmicity of 1 appeared, however, less signif
icant. The need for refined assay systems to fully delineate the importance
of rhythmical activity of 1 with respect to biological rhythmicity is brie
fly discussed. (C) 2000 Elsevier Science B.V. All rights reserved.