A rare PCR-RFLP within intron 2 of the porcine TSHB gene

Based on cDNA sequence of the porcine TSHB (thyroid stimulating hormone, be ta subunit) gene a fragment encompassing a part of exon 2 and exon 3 and in tervening intron was amplified using polymerase chain reaction (PCR). The P CR fragment was sequenced and the sequence was deposited in EMBL/GenBank/DD BJ databases (AJ131849). Of investigated pig breeds (Landrace, Large White, Duroc, Hampshire. Czech Meat Pig, Pietrain, and Meishan) a MnlI PCR-RFLP w as observed only in 2 of 11 Meishan pigs (frequencies A = 0.09, B = 0.91). TSHB was mapped by linkage analysis on chromosome 4 as follows: S0073 - 3.3 - ATP1A1 - 12.9 - EAL - 3.2 - NGFB - 2.3 - TSHB - 11.1 - SW2435 (distances given in Kosambi cM).