A quantitative enzyme-linked immunosorbent assay (ELISA) for the immunodiagnosis of neurocysticercosis using a purified fraction from Taenia solium cysticerci

Authors
da Silva, ADT Quagliato, EMAB Rossi, CL
Citation
Adt. Da Silva et al., A quantitative enzyme-linked immunosorbent assay (ELISA) for the immunodiagnosis of neurocysticercosis using a purified fraction from Taenia solium cysticerci, DIAG MICR I, 37(2), 2000, pp. 87-92
Citations number
39
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
ISSN journal
07328893 → ACNP
Volume
37
Issue
2
Year of publication
2000
Pages
87 - 92
Database
ISI
SICI code
0732-8893(200006)37:2<87:AQEIA(>2.0.ZU;2-I
Abstract
A quantitative enzyme-linked immunosorbent assay (ELISA) for the immunodiag nosis of neurocysticercosis is described. The ELISA was standardized using a purified Taenia solium cysticerci fraction (PCF) obtained by ion exchange chromatography. The ELISA using PCF (PCF-ELISA) and a qualitative ELISA us ing a whole extract from T. solium cysticerci (WEC-ELISA) were used to scre en for cysticercus-specific IgG antibodies in cerebrospinal fluid (CSF) sam ples fi om 57 patients with neurocysticercosis and 50 patients with other n eurologic disorders. The sensitivity of both assays was 95%, whereas the sp ecificities of PCF-ELISA and WEC-ELISA were 100% and 92%, respectively. The excellent sensitivity and specificity of the PCF-ELISA make this assay a p otentially useful tool in screening for antibodies against T. solium cystic erci. (C) 2000 Elsevier Science Inc. All rights reserved.