Separation of naphthalene-2,3-dicarboxaldehyde-derivatized-substance P andits metabolites by micellar electrokinetic chromatography

This paper describes the development of analytical methodology for the sepa ration of naphthalene-2,3-dicarboxaldehyde (NDA)-derivatized substance P (C BI-SP) and five lysine-containing metabolites by micellar electrokinetic ch romatography (MEKC). The effect of surfactant composition and organic modif iers on the separation was investigated. The final separation buffer consis ted of 80 mM sodium cholate in 50 mu N-tris (hydroxymethyl)methyl-2-aminoet hanesulfonic acid (TES), pH 7. All six lysine-containing peptides were sepa rated under these conditions.