An optimized microtiterplate assay to detect acetylcholinesterase activityin individual Chironomus riparius Meigen

Authors
Fisher, TC Crane, M Callaghan, A
Citation
Tc. Fisher et al., An optimized microtiterplate assay to detect acetylcholinesterase activityin individual Chironomus riparius Meigen, ENV TOX CH, 19(7), 2000, pp. 1749-1752
Citations number
25
Categorie Soggetti
Environment/Ecology
Journal title
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
ISSN journal
07307268 → ACNP
Volume
19
Issue
7
Year of publication
2000
Pages
1749 - 1752
Database
ISI
SICI code
0730-7268(200007)19:7<1749:AOMATD>2.0.ZU;2-5
Abstract
A sensitive microtiter plate assay to detect acetylcholinesterase activity in fourth-instar Chironomus riparius (Meigen) larvae was developed and opti mized to allow the measurement of pesticide inhibition in individuals. Opti mal conditions, including enzyme concentration, pH, and substrate concentra tion, allow the sensitive measurement of changes in activity following expo sure to the organophosphate insecticide chlorpyrifos. Significant reduction s in activity were observed at pesticide doses of 0.05, 0.1, 1.0, and 5 mg/ L following a 48-h exposure.