Lb. Christiansen et al., In vivo comparison of xenoestrogens using rainbow trout vitellogenin induction as a screening system, ENV TOX CH, 19(7), 2000, pp. 1867-1874
Vitellogenin induction in rainbow trout after intraperitoneal injection of
test compounds was investigated. Vitellogenin quantification was performed
by a direct sandwich enzyme-linked immunosorbant assay (ELISA) based on pol
yclonal, affinity purified antibodies. The system was used as a short-term
in vivo screen for estrogenicity and as a tool for establishing the priorit
y of compounds to be tested by water exposure of fish. Representatives from
the group of xenoestrogens, bisphenols, phthalates, and pesticides were te
sted. The relative potency order bisphenol A > bisphenol A dimethacrylate,
o,p'-DDT > butylbenzylphthalate was established, while no significant vitel
logenin response was seen with dibutylphthalate, tetrabromobisphenol A, and
methoxychlor. Of these, tetrabromobisphenol A has not previously been test
ed for estrogenicity in vivo. Earlier findings of diminished estrogenic act
ivity of ortho-substituted compounds was extended to bisphenols since ortho
-bromination of bisphenol A abolished the activity of the parent molecule.