A methodology to test the toxicity of marine and estuarine sediments was de
veloped using postlarval organisms of the marine shrimp Penaeus schmitti an
d P. paulensis. The tests were conducted in aquariums with a water feedback
system and a sediment layer of 2 cm. The postlarvae exposure time to the s
ediments was 10, 28, and 52 d. The tested sediments were collected in conta
minated sites of Guanabara and Sepetiba Bays and at the reference site of I
lha Grande inlet in Rio de Janerio, Brazil. The toxicity of the sediments w
as evidenced with exposures of 28 d or longer. The sediment from Saco do En
genho (Sepetiba Bay) was the most toxic, affecting the survival of both P.
schmitti and P. paulensis. The sediment from Guanabara Bay was toxic to P.
schmitti, affecting its survival after 28 d of exposure.