Production and characterization of antibodies directed against the human melatonin receptors Mel-1a (mt1) and Mel-1b (MT2)

Authors
Angeloni, D Longhi, R Fraschini, E
Citation
D. Angeloni et al., Production and characterization of antibodies directed against the human melatonin receptors Mel-1a (mt1) and Mel-1b (MT2), EUR J HIST, 44(2), 2000, pp. 199-204
Citations number
21
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
EUROPEAN JOURNAL OF HISTOCHEMISTRY
ISSN journal
1121760X → ACNP
Volume
44
Issue
2
Year of publication
2000
Pages
199 - 204
Database
ISI
SICI code
1121-760X(2000)44:2<199:PACOAD>2.0.ZU;2-A
Abstract
We report the production of polyclonal antibodies directed against the huma n melatonin receptors Mel-1a (mt1) and Mel-1b (MT2) by means of antigenic s ynthetic peptides with sequences unique to these proteins. Immunostaining o n NIH3T3 cells stably transfected with Mel-1a and Mel-1b cDNA gave intense reactions. Neither the preimmune serum nor cross-tested antisera showed any reactivity. These polyclonal antibodies will be essential immunocytochemic al tools to study the human melatonin receptors distribution at subcellular level.