Simultaneous monitoring of vascular contractility, intracellular pH and intracellular calcium in isolated rat mesenteric arteries; effects of weak bases

Authors
Otter, D Austin, C
Citation
D. Otter et C. Austin, Simultaneous monitoring of vascular contractility, intracellular pH and intracellular calcium in isolated rat mesenteric arteries; effects of weak bases, EXP PHYSIOL, 85(3), 2000, pp. 349-351
Citations number
16
Categorie Soggetti
Physiology
Journal title
EXPERIMENTAL PHYSIOLOGY
ISSN journal
09580670 → ACNP
Volume
85
Issue
3
Year of publication
2000
Pages
349 - 351
Database
ISI
SICI code
0958-0670(200005)85:3<349:SMOVCI>2.0.ZU;2-2
Abstract
We report the first simultaneous measurements of pH(i), [Ca2+](i) and tensi on, upon alteration of pH(i), in isolated rat mesenteric arteries loaded wi th both carboxy SNARF and indo-1. In these vessels (pre-contracted by 30 mM KCl) alterations of pH(i), by addition and subsequent washout of weak base s, produced complicated effects on tone. Although the changes in contractil ity did not mirror the changes in pH(i) they were, at all times, accompanie d by parallel changes in [Ca2+](i).