A survey was conducted in Prince William Sound (PWS) in May 1996, to study
the volume backscattering of zooplankton using a 420 kHz digital sonar and
a multiple opening-closing net environmental sampling system (MOCNESS). Whi
le the digital sonar provided continuous fine-scale volume scattering stren
gth measurements, the MOCNESS samples provided zooplankton species, size, a
nd coarse-scale estimates of density and distribution. This catch informati
on was applied to acoustic scattering models to predict zooplankton volume
backscattering strength. By least-squares fitting the predicted backscatter
values with the measured acoustic backscatter, we determined the reflectio
n coefficients for Neocalanus, pteropods, and eupausiids to be 0.0178, 0.15
33, and 0.0341, respectively. By applying these reflection `coefficients an
d the measured animal sizes to the same acoustic models, we were able to ca
lculate target strengths (TSs) for these tars, which were then applied to t
he volume scattering information, yielding estimates of the absolute densit
ies of those selected species along continuous transects inside and outside
PWS, The comparison of these density values with the density estimates fro
m the MOCNESS showed reasonable agreement, although the distribution of aco
ustic values was much wider than the MOCNESS due to finer sampling. Since r
eflection coefficients vary from species to species, it is inappropriate to
borrow this value from another species based on similarity. (C) 2000 Elsev
ier Science B.V. All rights reserved.