Establishment and usefulness of an anti-human CD57 IgG1 monoclonal antibody

Citation
J. Tajima et al., Establishment and usefulness of an anti-human CD57 IgG1 monoclonal antibody, IMMUNOL LET, 72(3), 2000, pp. 159-162
Citations number
12
Categorie Soggetti
Immunology
Journal title
IMMUNOLOGY LETTERS
ISSN journal
01652478 → ACNP
Volume
72
Issue
3
Year of publication
2000
Pages
159 - 162
Database
ISI
SICI code
0165-2478(20000601)72:3<159:EAUOAA>2.0.ZU;2-1
Abstract
In the present study we established a new monoclonal antibody, JNK-1, which recognizes all cells recognized by CD57/HNK-1 mAb. JNK-1 and CD57 mAbs inh ibited the binding of each other, suggesting that the molecules they recogn ize are either identical or sufficiently close to cause steric hindrance in the binding assay. JNK-1 mAb detected the 110-kDa protein, which is identi cal to the protein recognized by CD57/HNK-1 mAb in Western immunoblot analy sis combined with immunoprecipitation. Therefore, JNK-1 mAb appears to reco gnize homogeneous molecules identified by the currently available CD57 mAb. Notably, JNK-1 mAb is composed of mouse IgG1 heavy chains, and thus can be used easily in immunoprecipitation, which cannot easily be performed with the available CD57 mAb because it is an IgM isotype. Thus. JNK-1, which is an IgG isotype, may present a useful tool to elucidate the CD57 protein. (C ) 2000 Elsevier Science B.V. All rights reserved.