Epitope mapping on N-terminal region of Taenia solium paramyosin

Epitope mapping of the amino-terminal 20aa sequence from Taenia solium para myosin (TPmy), an immunodominant protein involved in the complex host-paras ite relationship in human and porcine cysticercosis is reported. A 12-mer r andom peptide phage display library was screened with antibodies raised aga inst a synthetic peptide corresponding to the amino-terminal 20aa sequence of TPmy, its highly immunodominant region. In total, 57 clones isolated in two panning conditions were analyzed, of which a single group of 14 sequenc es found in 25 clones shared a consensus motif showing structural similarit y with the antigen Arg10-Thr16 region. (C) 2000 Elsevier Science B.V. All r ights reserved.