Protection of DNA from radiation-induced double-strand breaks: influence of replication and nuclear proteins

Citation
A. Sak et al., Protection of DNA from radiation-induced double-strand breaks: influence of replication and nuclear proteins, INT J RAD B, 76(6), 2000, pp. 749-756
Citations number
30
Categorie Soggetti
Experimental Biology
Journal title
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
ISSN journal
09553002 → ACNP
Volume
76
Issue
6
Year of publication
2000
Pages
749 - 756
Database
ISI
SICI code
0955-3002(200006)76:6<749:PODFRD>2.0.ZU;2-1
Abstract
Purpose: To study the protective effect of histone and non-histone proteins on double-strand break (dsb) induction in replicating S-phase DNA as well as bulk DNA of plateau phase human tumour cells. Materials and methods: Induction of dsb was studied in two human adenocarci noma cell lines: Colo320HSR and MCF-7. To assess the influence of chromatin structure on radiation-induced DNA dsb, different nuclear preparations of cells, either continuously labelled with C-14 or pulse labelled with H-3, w ere assessed by pulsed-field gel electrophoresis (PFGE). Results and conclusions: Stepwise removal of DNA-bound proteins from the ch romatin increased the amount of radiation-induced dsb in both cell lines. H owever, the protective effect of DNA-associated proteins on dsb induction w as significantly reduced in DNA of replicating S-phase cells compared with bulk DNA of plateau phase cells. These data show that proteins associated w ith the DNA have a different protective effect on radiation-induced dsb, re ndering replicating DNA with open chromatin structure more sensitive to dsb induction by ionizing radiation.