The effect of evening primrose oil on the radiation response and blood flow of mouse normal and tumour tissue

Purpose: To investigate the effect of the oral administration of evening pr imrose oil on the radiation response and the blood flow of normal tissue an d a tumour in BALB/c mice. Methods and materials: Aliquots of evening primrose oil were fed to BALB/c mice daily and the radiation response of the skin was assessed by the deter mination of ED50 values for the incidence of moist desquamation, using prob it analysis. Tumour radiosensitivity was investigated by determining the gr owth delay caused by irradiation of a transplantable rhabdomyosarcoma. The (RbCl)-Rb-86 uptake technique was used to determine the blood flow in norma l foot and tumour tissue. The fatty-acid content of red blood cells, plasma and tumour tissue was measured using gas chromatography. Results: Daily evening primrose oil dietary supplementation reduced the sen sitivity of skin to radiation-induced moist desquamation and prevented the radiation-associated increase in blood flow that was observed in this tissu e. NO modification of tumour blood flow or of tumour sensitivity to radiati on resulted from evening primrose oil supplementation of mice. Evening prim rose oil supplementation resulted in changes in plasma levels of linoleic a cid (LA), gamma-linolenic acid (GLA), dihomo-gamma-linolenic acid (DGLA) an d arachidonic acid (AA). These changes were contingent on whether the mice had been irradiated or not. In red blood cells evening primrose oil supplem entation increased the GLA level of unirradiated mice and the LA level at 2 0 days after irradiation. There were no changes in tumour fatty-acid levels as a result of evening primrose oil treatment. Conclusions: Daily evening primrose oil supplementation reduced the sensiti vity of skin to radiation-induced moist desquamation but did not alter tumo ur sensitivity to radiation.