Eight strains of Salmonellae were incubated in TSB culture medium at 37 deg
rees C for 24 h. Volatile compounds derived from the bacteria were collecte
d using solid-phase microextraction fibers and then applied to gas chromato
graphy (GC). Similarity analysis of the GC patterns thus obtained could sep
arate these strains on principal component similarity (PCS) scattergrams. F
ive major food-related pathogenic bacteria and 10 other bacteria (including
one Salmonella strain)were also classified by growing in the same medium.
It is then proposed to utilize this approach to improve the GC/PCS method o
f Nakai et al. [Nakai, S.; Wang, Z. H.; Dou, J.; Nakamura, S.; Ogawa, M.; N
akai, E.; Vangerstoep, J. J. Agric. Food Chem. 1999, 47, 576-583] that has
been developed for screening safe foods by detecting bacteria contaminated
foods. Inoculating food samples pre-enriched through preliminary incubation
into a culture medium and then subjecting to the GC/PCS method after secon
dary incubation enhances the detectability of pathogenic bacteria.