F. Toribio et al., Comparison of different commercial solid-phase extraction cartridges used to extract heterocyclic amines from a lyophilised meat extract, J CHROMAT A, 880(1-2), 2000, pp. 101-112
Heterocyclic amines are a group of potent mutagenic compounds which are gen
erated when muscle meat is cooked. Since they are possible human carcinogen
s, these mutagens have received considerable attention in recent years, and
several analytical techniques have been developed for their quantification
. Although the purification step is one of the most important, there are a
great number of variables influencing the recovery of the amines, especiall
y when real samples are analysed. In this work we studied the influence of
sample spiking mode on the recoveries. Furthermore, on the basis of a previ
ously developed clean-up method, the effect of changing commercial source a
nd structure of the sorbents used in two solid-phase extraction steps was e
xamined. This purification method was applied to the quantification of the
heterocyclic aromatic amines present in a lyophilised meat extract by means
of Liquid chromatography-mass spectrometry. (C) 2000 Elsevier Science B.V.
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