Determination of aldehydes in food by high-performance liquid chromatography with biosensor coupling and micromembrane suppressors

A high-performance liquid chromatography system for the determination of al dehydes in food was developed incorporating an Cation MicroMembrane Suppres sor (CMMS) and enzyme reactors packed with VA-Epoxy on which aldehyde dehyd rogenase from bakers yeast and NADH oxidase from Bacillus licheniformis wer e immobilized. The method was based on the principle that the separation ef ficiency of HPLC is combined with the sensitivity of electrochemical detect ion and the specificity of enzymes. Main attention was directed to the dete rmination of 5-hydroxymethyl-2-furaldehyde and 2-furaldehyde, the occurence of which is an indication of quality deterioration in several food product s. The efficiency of the method has been shown by the analysis of honey, co ffee and related beverages, refreshments, sherry, port, dry fruits and brea kfast cereals, (C) 2000 Elsevier Science B.V. All rights reserved.