Separation and detection techniques for peptides and proteins in stabilityresearch and bioanalysis

In this paper, a brief overview of the most commonly used methods for the s eparation and analysis of peptides and proteins in stability and bioanalysi s studies is presented. To investigate the physical stability of peptides a nd proteins, size-exclusion chromatography;ind electrophoretic separation t echniques are being used, apart from several other methods. To determine th e chemical stability of these compounds, separation systems are also import ant, with informative detection modes, such as various spectroscopic detect ions, electrochemical detection and mass spectrometric detection. For the b ioanalysis of peptides, separation is the most important factor, while the detection must be done at the highest possible level of sensitivity. (C) 20 00 Elsevier Science B.V. All rights reserved.