Capillary gel electrophoresis: separation of major erythrocyte membrane proteins

ii new separation method of human erythrocyte membrane proteins by sodium d odecyl sulfate capillary gel electrophoresis (SDS-CGE) is described. In thi s method, a replaceable gel matrix was used. Seven major erythrocyte membra ne proteins, or-and P-spectrin, ankyrin 2.1, band 3 (anion-exchanger), 4.1a and b, and 4.2 (pallidin), were separated and identified by SDS-CGE method . High reproducible migration times of these proteins (inter-assay coeffici ents of variation less than 2%), as well as quantification (inter-assay coe fficients of variation less than 11%) were obtained. This new SDS-CGE metho d may provide important diagnostic evidence for hereditary spherocytosis. I t can be a powerful diagnostic tool in place of SDS polyacrylamide gel elec trophoresis for erythrocyte membrane protein analysis. (C) 2000 Elsevier Sc ience B.V. All rights reserved.