Tr. Mackle et al., Evaluation of whole blood and plasma in the interorgan supply of free amino acids for the mammary gland of lactating dairy cows, J DAIRY SCI, 83(6), 2000, pp. 1300-1309
We investigated the contribution of plasma and red blood cells to amino aci
d (AA) supply for milk protein synthesis during a combination of treatments
that included abomasal infusion of casein and AA and utilization of a hype
rinsulinemic-euglycemic clamp. Treatments resulted in substantial differenc
es in circulating concentrations of AA, mammary uptake of AA, and rates of
milk protein synthesis. Arterial concentrations of all AA in plasma were hi
ghly correlated with that of whole blood. Concentrations of AA in red blood
cells were either higher (Asn+Asp, Gly, His, Leu, Met, Om, Ser, Tau, Thr,
and Tyr), lower (Ala, Arg, Cit, Cys, lie, and Val), or similar (Gln+Glu, Ph
e, and Pro) to that of plasma. Arteriovenous difference measurements demons
trated that interorgan transfer of AA to the mammary gland was primarily by
plasma. There was little involvement of red blood cells except for small q
uantities of Leu, Met, and Thr to the mammary gland; this contribution was
greatest for Met and accounted for 14% of the total mammary uptake. Counter
current transport of Gln+Glu, Asn+Asp, and Pro was also evident where these
AA were extracted from plasma, but were released into red blood cells as b
lood passed through the mammary gland. This net influx of Gln+Glu, Asn+Asp,
and Pro into red blood cells was equivalent bo 26, 17, and 30% of their ma
mmary uptake from plasma. Overall, the interorgan transport of free AA for
the mammary gland was predominantly by plasma, and red blood cells were lim
ited to minor contributions in mammary uptake for a few BA. Furthermore, ar
teriovenous differences of essential AB across the mammary gland were highl
y correlated between plasma and whole blood.