A detailed understanding of the complexity of root canal systems is imperat
ive to ensure successful root canal preparation. The aim df this study was
to evaluate the potential and accuracy of a three-dimensional, non-destruct
ive technique for detailing root canal geometry by means of high-resolution
tomography. The anatomy of root canals in 12 extracted human maxillary mol
ars was analyzed by means of a micro-computed tomography scanner (mu CT, cu
bic resolution 34 pm). A special mounting device facilitated repeated preci
se repositioning of the teeth in the mu CT. Surface areas and volumes of ea
ch canal were calculated by triangulation, and means were determined. Model
-independent methods were used to evaluate the canals' diameters and config
uration. The calculated and measured volumes and the areas of artificial ro
ot canals, produced by the drilling of precision holes into dentin disks, w
ere well-correlated. Semiautomated repositioning of specimens resulted in n
ear-perfect matching (< 1 voxel) when outer canal contours were assessed. R
oot canal geometry was accurately assessed by this innovative technique; th
erefore, variables and indices presented may serve as a basis for further a
nalyses of root canal anatomy in experimental endodontology.