Controlled electrocatalysis by microperoxidase-11 and Au-nanoparticle superstructures on conductive supports

Three-dimensional superstructures, multilayer-arrays consisting of Au-nanop articles (13 +/- 1 nm) and crosslinked by microperoxidase-11 (MP-11), were assembled on transparent ITO conductive glass supports. The structures of t he assemblies were confirmed by spectroscopic and electrochemical analyses, which revealed that each Au-particle is associated with ca. 50 MP-11 molec ules. The multilayered MP-11/Au-particle systems act as electrocatalysts fo r the reduction of H2O2. The number of MP-11 / Au-particle layers associate d with the electrode controls the resulting electrocatalytic currents. The controllable number of MP-11 / Au-particle layers associated with the elect rode enables the control of the effectiveness of the electrocatalytic proce ss and tuning of the sensitivity of the H2O2-sensing interface. (C) 1999 El sevier Science S.A. All rights reserved.