Adhesion of human osteoblasts on root-end filling materials

Adhesion of human osteoblasts to root-end filling materials (mineral trioxi de aggregate (MTA), IRM, composite, and amalgam) was observed by scanning e lectron microscopy, Root-end filling materials were inserted into 96-well f lat-bottomed plates and condensed to disks of similar to 1 mm thick and the same diameter as the wells. After the disks were set, they were placed in the bottom of Nunc four-well culture plates at one disk per well. Then huma n osteoblasts were seeded into the wells at 1.5 x 10(5) cells per well, Aft er 1 day in culture the disks of root-end filling materials along with cell s grown on their surface were examined with a scanning electron microscopy, Results showed that osteoblasts attached and spread on MTA and composite b y forming a monolayer, Osteoblasts also attached on amalgam, but with few c ells spreading. In the presence of IRM, osteoblasts appeared rounded with n o spreading. These results indicate that osteoblasts have a favorable respo nse to MTA and composite resin compared with IRM and amalgam.