Microwave irradiated canine and feline tissues: Part 1. Morphologic evaluation

Authors
Citation
Td. Lemire, Microwave irradiated canine and feline tissues: Part 1. Morphologic evaluation, J HISTOTECH, 23(2), 2000, pp. 113-120
Citations number
10
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
JOURNAL OF HISTOTECHNOLOGY
ISSN journal
01478885 → ACNP
Volume
23
Issue
2
Year of publication
2000
Pages
113 - 120
Database
ISI
SICI code
0147-8885(200006)23:2<113:MICAFT>2.0.ZU;2-E
Abstract
A variety of canine and feline tissues collected during endoscopic/wedge bi opsy procedures or during routine necropsies were microwave irradiated for 30 to 60 sec to a solution temperature 58-60 degrees C, in either phosphate buffered saline (PBS), a 50/50 mix of PBS and ethanol, or Klotz solution. A duplicate set of tissues from each animal was collected in 10% neutral bu ffered formalin (NBF) as a control. Ail tissues were routinely processed, p araffin embedded, and stained with hematoxylin and eosin. Histologic compar isons were made between canine and feline tissues stabilized by microwave i rradiation and tissues from the same animals routinely fixed in 10% NBF for 24 hr. Canine and feline tissues collected in physiologic buffered saline or Klotz solution and immediately irradiated demonstrated minimal artifactu al changes in histologic appearance. Thus, microwave irradiation, with PBS or Klotz solution, can be a viable alternative to overnight techniques rout inely used in most veterinary laboratories and may be used in lieu of forma lin fixation to provide same day histopathologic diagnoses.