BIOMARKER EVIDENCE OF DNA OXIDATION IN LUNG-CANCER PATIENTS - ASSOCIATION OF URINARY 8-HYDROXY-2'-DEOXYGUANOSINE EXCRETION WITH RADIOTHERAPY, CHEMOTHERAPY, AND RESPONSE TO TREATMENT

Ratios of urinary 8-hydroxy-2'-deoxyguanosine to urinary creatinine (8 -OHdG/creatinine) have been considered as a good biological indicator of DNA oxidation, Urinary 8-OHdG/creatinine levels of lung cancer pati ents were evaluated by enzyme-linked immunosorbent assay using a monoc lonal antibody N45.1 during radiotherapy and chemotherapy, An increase in urinary 8-OHdG/creatinine was found in non-small-cell carcinoma (n on-SCC) patients during the course of radiotherapy, SCC patients showe d higher levels of urinary 8-OHdG/creatinine than the controls. Furthe rmore, SCC patients with complete or partial response to the chemother apy showed a significant decrease in urinary 8-OHdG/creatinine while p atients with no change or progressive disease showed an increase. (C) 1997 Federation of European Biochemical Societies.