Studies on urinary metabolites of perlolyrine by stable isotope tracer method

After oral administration of perlolyrine and deuterated perlolyrine, the ra t urines were collected, hydrolyzed with glucuronidase, basified with NaHCO 3-Na2CO3, extracted with ethyl ether-iso-propyl alcohol. The organic phases (neutral and basic fractions) were concentrated for conversion to TMS deri vatives. The aqueous phase were acidified with sulfuric acid, taken to dryn ess under a nitrogen stream, extracted with methanol (water soluble acidic fractions) and concentrated for conversion to TMS derivatives. The TMS deri vatives were determined by gas chromatography-mass spectrometry (GC-MS). Pe rlolyrine and one metabolite were found from the neutral and basic fraction s, and two different metabolites were found from the water soluble acidic f ractions. It was proposed that the major possible metabolic pathways of per lolyrine were the hydroxylation of perlolyrine and the oxidition of its hyd roxylmethyl group.