Identification of an immunogenic 18-kDa protein of Helicobacter pylori by alkaline phosphatase gene fusions

The use of alkaline phosphatase fusion methodology to identify Helicobacter pylori exported proteins enabled the identification of an open reading fra me (ORF) encoding a highly immunogenic, previously uncharacterised exported protein, The predicted aminoacid sequence displays a typical N-terminal si gnal peptide and contains regions of C-terminal hydrophobicity consistent w ith a membrane-associated protein, Southern blot analysis revealed that the gene encoding the protein was absent in several Helicobacter spp, and a co mbination of PCR and sequence analysis of the amplified gene showed that it is highly conserved amongst isolates of H, pylori. To obtain pure recombin ant protein, the gene encoding the protein was cloned and expressed as a be ta-galactosidase (beta-gal) fusion in Escherichia coli and the protein was purified by affinity chromatography and proteolytic cleavage of the beta-ga l portion. The purified protein, which has an apparent mel. wit of 18 kDa, was recognised by antibody present in 71% of sera from patients infected wi th H, pylori, but in only 16% of sera from patients with unrelated or no ga strointestinal disease, by Western blot assays. These results indicate that the 18-kDa protein from H, pylori is immunogenic and is expressed ill vivo .