In this work we present a simple, non-contact method for characterizing the
depth of deep zero-order diffraction gratings using standard visible optic
s. Form birefringence exhibited by zero-order structures alters the polariz
ation state of light transmitted through them. The amount of change is depe
ndent on several factors including the depth of the grating grooves. By rec
ording the polarization changes of laser light transmitted through the samp
le we demonstrate how the depth of any grating with a known pitch may be de
termined. Results for many different gratings have been analysed and show g
ood agreement with groove depths measured from scanning electron micrograph
s. From these results a universal relationship between the amount of polari
zation change and the depth of the grating grooves has been determined.