Slow deactivation kinetics of NMDA receptors containing NR1 and NR2D subunits in rat cerebellar Purkinje cells

1. We have examined the deactivation kinetics of native Iv-methyl-D-asparta te receptors (NMDARs) containing NR1 and NR2D subunits by patch-clamp recor ding from Purkinje cells in cerebellar slices from young rats. 2. NMDAR-mediated whole-cell currents were elicited in response to bath app lication of 20 mu M NMDA and 50 mu M glycine. The NMDAR-mediated currents w ere small, with an average whole-cell conductance of approximately 750 pS. 3. Following the rapid application of brief pulses (1-10 ms) of 1 mM glutam ate to outside-out membrane patches, we observed a low-conductance type of single-channel activity which lasted up to 30 s after the removal of agonis t. 4. Analysis of individual channel openings revealed asymmetry of transition s between the main- and subconductance states - a characteristic of NR1/NR2 D-containing NMDARs. The averaged macroscopic current exhibited a decay tim e course which was well described by a single exponential function with a t ime constant of similar to 3 s. 5. We conclude that native NR1/NR2D-containing NMDARs, like their recombina nt counterparts, display very slow deactivation kinetics. This feature shou ld provide a means for identification of these receptors at synapses, and i ndicates that they do not contribute to the synaptic NMDAR currents so far described.