The micro-organisms associated with malt have an important influence on qua
lity parameters. Although it was not possible to eliminate the growth of mi
cro-organisms entirely during the malting process, it was possible to contr
ol particular groups by applying some simple procedures. A three steep sche
dule was associated wit increased fungal growth which wa snot observed when
a two steep schedule was used. Fungal proliferation could also be reduced
by the use of hop beta-acids. The use of sodium hypochlorite, potassium hyd
roxide, hydrochloric acid and hop beta-acids reduced or eliminated E. coli.
The most effective means of controlling total micro-flora was an alternate
acid/alkaline wash.
There was no evidence that a correlation existed between growth site and mi
crobial loading for the years studied. Furthermore, whilst many of the barl
ey associated micro-organisms were able to secrete brewing related enzymes,
the activities of these were too low to have any significant effect during
mashing.