Effect of leukocyte-endothelial adhesion antagonism on neutrophil migration and neurologic outcome after cortical trauma

Background: Administration of anti-CD11B, a monoclonal antibody directed ag ainst the leukocyte adhesion molecule CD11B, results in decreased neutrophi l infiltration into injured tissue after experimental ischemia, We determin ed the effect of anti-CD11B administration on neutrophil migration and neur ologic functioning after experimental cortical trauma. Methods: Injuries were produced by a pneumatic impactor. Treatment animals received anti-CD11B after injury, Neurologic functioning was quantitated at 1, 12, and 24 hours after injury. Neutrophil migration was assessed with t he myeloperoxidase assay. Results: Neutrophil influx was increased in injured cortex after trauma. An ti-CD11B significantly reduced neutrophil influx. There was no significant improvement in neurologic functioning after MAb administration. Conclusions: These results show there is marked neutrophil response to inju ry as produced with the pneumatic contusion model. This migration may be si gnificantly attenuated by administration of a anti-CD11B.