Expression of cyclin-dependent kinase 6 (cdk6) and frequent loss of CD44 in nasal-nasopharyngeal NK/T-Cell lymphomas: Comparison with CD56-negative peripheral T-cell lymphomas

Lymphomas involving the nasal and nasopharyngeal region mainly include CD56 -positive natural killer (NK)TT-cell lymphomas, CD56-negative peripheral T- cell lymphomas (PTL), and B-cell lymphomas. Among these, the CD56-positive lymphoma, presumably of an NWT-cell nature, is frequently seen in Asian, Me xican, and South American patients. NK cells are proposed to be closer deve lopmentally to T cells than to other lymphoid cells, because bipotential co mmon progenitor cells of NWT-cell lineage have been isolated. In this study , we collected 47 cases of nasal lymphoma and investigated the phenotypic d ifference between NWT-cell lymphoma and Pn. by examining the pattern of the developmentally differentially expressed molecules cdk6 (cyclin-dependent kinase 6), CD44, CD117, and by examining the rearrangement of the T-cell re ceptor gene (TcR-GR). cdk6, an essential regulator of the cell cycle in G(1 ) progression, was over-expressed in a subset of cortical thymocytes, but a bsent in mature thymocytes. In contrast, CD44, a glycosylated adhesion mole cule, was absent in cortical thymocytes, but present in mature thymocytes a nd peripheral activated T cells. We found both over-expression of nuclear c dk6 (n-cdk6) and frequent absence of CD44 in nasal GD56-positive NK/T-cell lymphomas, in contrast to most nasal CD56-negative PTL, which were CD44-imm unoreactive with weak or no expression of n-cdk6. Almost all tested cases o f NWT-cell lymphoma displayed a germ-line configuration of TcR, without evi dence of gene rearrangement. Thus, there seems to be a useful distinction b etween the classical Nt(TT type of nasal lymphoma (CD56(+)/n-cdk6(+)/CD44(- )/TcR-GR(-)) and PTL (CD56(-)/n-cdk6(-)/CD44(+)/TcR-GR(+)) involving the na sal region. The presence of Epstein-Barr virus does not seem to be a good m arker for distinguishing between NWT lymphoma and PTL involving the nasal r egion.