Immunodetection of adenylyl cyclase protein in tissues

Citation
Y. Ishikawa et al., Immunodetection of adenylyl cyclase protein in tissues, MOL C ENDOC, 162(1-2), 2000, pp. 107-112
Citations number
14
Categorie Soggetti
Endocrinology, Nutrition & Metabolism
Journal title
MOLECULAR AND CELLULAR ENDOCRINOLOGY
ISSN journal
03037207 → ACNP
Volume
162
Issue
1-2
Year of publication
2000
Pages
107 - 112
Database
ISI
SICI code
0303-7207(20000425)162:1-2<107:IOACPI>2.0.ZU;2-L
Abstract
Immunodetection of the adenylyl cyclase isoforms has been difficult in tiss ues because of its low quantity of protein expression. We have developed a one-step cellular protein purification method that enables a simple immunod etection of the adenylyl cyclase isoforms. The type I isoform was detected exclusively in the brain. The type III isoform was detected in the brain an d lungs. Further, the protein expression of type III adenylyl cyclase in lu ngs changed ontogenically and was the lowest in neonates. Thus, the compari son of the amount of certain adenylyl cyclase isoforms protein in each tiss ue is now feasible using our method. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.