Immunodetection of adenylyl cyclase protein in tissues

Immunodetection of the adenylyl cyclase isoforms has been difficult in tiss ues because of its low quantity of protein expression. We have developed a one-step cellular protein purification method that enables a simple immunod etection of the adenylyl cyclase isoforms. The type I isoform was detected exclusively in the brain. The type III isoform was detected in the brain an d lungs. Further, the protein expression of type III adenylyl cyclase in lu ngs changed ontogenically and was the lowest in neonates. Thus, the compari son of the amount of certain adenylyl cyclase isoforms protein in each tiss ue is now feasible using our method. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.