Multiplicity of site-specific DNA methyltransferases of the BstF5I restriction-modification system

Authors
Belavin, PA Netesova, NA Gutorov, VV Golikova, LN Abdurashitov, MA Gonchar, DA Degtyarev, SK
Citation
Pa. Belavin et al., Multiplicity of site-specific DNA methyltransferases of the BstF5I restriction-modification system, MOL BIOL, 34(3), 2000, pp. 378-381
Citations number
15
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR BIOLOGY
ISSN journal
00268933 → ACNP
Volume
34
Issue
3
Year of publication
2000
Pages
378 - 381
Database
ISI
SICI code
0026-8933(200005/06)34:3<378:MOSDMO>2.0.ZU;2-A
Abstract
A fragment located downstream of the genes for DNA methyltransferases of Ba cillus stearothermophilus F5 (M.BstF5I-I and M.BstF5I-2) was sequenced. The fragment contains a gene for another methylase, M.BstF5I-3, structurally a nd functionally similar to the N-terminal domain of M.FokI. Thus, in contra st to other restriction-modification systems, the BstF5I system includes th ree methylases, two being homologous to the individual M.FokI domains.