LINKAGE MAPPING OF THE TUMOR-NECROSIS-FACTOR RECEPTOR-2 (TNFR2) GENE TO 1P36.2 USING THE SINGLE-STRAND CONFORMATION POLYMORPHISM TECHNIQUE

We identified two informative polymorphisms in the transcribed 3' untr anslated region of the tumor necrosis factor receptor 2 (TNFR2) gene u sing the polymerase chain reaction (PCR) with the single-strand confor mation polymorphism (SSCP) technique. These polymorphisms demonstrated Mendelian inheritance and were useful for linkage analysis. TNFR2 was very closely linked to the pronatriodilatin gene (PND), and the TNFR2 SSCP polymorphisms were much more informative than the restriction fr agment length polymorphisms currently available for the PND locus. Ln addition, we have demonstrated that genotyping could be performed with DNA obtained from paraffin-embedded tissue.