Double-resonance J-edited H-1-NMR detection of [6-C-13]-D-2-deoxyglucose uptake in glioma cells

The C6 methylene protons were selectively detected in H-1-NMR spectra of in tact glioma cells incubated with [6-C-13]-D-2-deoxyglucose ([6-C-13]-2dG), a C-13-enriched glucose analog that is suitable for monitoring glucose util ization in brain tumors. Spectral editing via H-1-C-13 scalar coupling was performed with twin spin-echo double resonance (T-SEDOR), a pulse sequence which combines chemical specificity and high sensitivity, requires no solve nt pre-saturation, and can easily be adapted to imaging protocols. This wor k demonstrates the suitability of the pulse sequence for monitoring [6-C-13 ]-2dG uptake in living cells in vitro, in spite of line-broadening and the occurrence of other strong signals in the spectral region of interest (3.5- 4.4 ppm). Copyright (C) 2000 John Wiley & Sons, Ltd.