Involvement of the glutathione S-conjugate compounds and the MRP protein in Tc-99m-tetrofosmin and Tc-99m-sestamibi uptake in glioma cell lines

The objective of this study was to compare the accumulation of Tc-99m-tetro fosmin and Tc-99m sestamibi in four grade IV glioma cell lines and to corre late their accumulation with the multidrug resistance of the cells. Tc-99m- tetrofosmin in all glioma cell lines showed slightly higher uptake and more efficient release beyond 150 min than Tc-99m-sestamibi and the retention o f both tracers in the cells was to a certain extend inversely proportional to their degree of multidrug resistance. The results obtained showed that t he efflux of both tracers was carried out only in part through the MRP/GS-X pump system, Tc-99m-tetrofosmin showed good potential as a marker of recur rent malignant glioma and in vivo studies are currently underway to confirm these observations. NUCL MED BIOL 27;3:299-307, 2000. (C) 2000 Elsevier Sc ience Inc. All rights reserved.