The effect of different supplements containing docosahexaenoic acid on plasma and erythrocyte fatty acids of healthy non-pregnant women

To find an appropriate dietary supplement for pregnant women that would enh ance their docosahexaenoic acid (DHA, 22:6n-3) status, without decreasing t he n-6 long-chain polyenes (LCP) status, both in plasma and in erythrocyte phospholipids, a dose-finding study was performed in healthy non-pregnant w omen. Six groups of non-pregnant women received daily over a 4-week period one of the following supplements: tuna fish oil (0.266g/d or 0.532 g/d DHA) , single cell-derived oils containing either DHA alone (0.285g/d or 0.570 g /d) or DHA and arachidonic acid (AA, 20:4n-6; 0.570 g/d DHA and 0.259 g/d A A). A control group received a placebo containing microcrystalline cellulos e. Fatty acids were analyzed in plasma, and erythrocyte phospholipids of th e blood samples collected weekly. After 4 weeks of supplementation, the lev els of DHA and n-6 LCP of the control group were unchanged. In the other gr oups, the levels of DHA were significantly and dose-dependently increased a s compared to those in the control group. Significant reductions were found in the levels of n-6 LCP, except in the group that received the mixture of DHA and AA enriched single cell oils. So, only this latter mixture was eff ective in increasing the levels of DHA and preventing an n-3 induced reduct ion in n-6 LCP. Whether this supplement effect will be the same in pregnant women needs further investigation. (C) 2000 Elsevier Science Inc.